The potential of Ethiopian medicinal plants to treat emergent viral diseases.

Ethiopians have deep-rooted traditions of using plants to treat ailments affecting humans and domesticated animals. Approximately 80% of the population continues to rely on traditional medicine, including for the prevention and treatment of viral diseases. Many antiviral plants are available to and widely used by communities in areas where access to conventional healthcare systems is limited. In some cases, pharmacological studies also confirm the potent antiviral properties of Ethiopian plants. Building on traditional knowledge of medicinal plants and testing their antiviral properties may help to expand options to address the global pandemic of COVID-19 including its recently isolated virulent variants and prepare for similar outbreaks in the future. Here, we provide an ethnobotanical and pharmacological inventory of Ethiopian medicinal plants that might contribute to the prevention and treatment of viral diseases. We identified 387 species, about 6% of Ethiopia's known flora, for which records of use by local communities and traditional herbalists have been documented for the treatment of viral diseases. We provide a framework for further investigation and development of this vital resource much anticipated to help combat emergent viral diseases along with existing ones in Ethiopia and elsewhere.

Forest products monetary contribution to households' income: A means to improve the livelihood of a low-income rural community in South Ethiopia.

Forest products and forest-based activities in Ethiopia play a substantial economic contribution to the livelihoods of rural households. Despite its pivotal role, empirical data are inadequate on forests' monetary contribution across a range of ecological locations and social situation, particularly for rural agrarian communities of the developing nations, like Ethiopia. We estimated the economic contributions of forest products and forest-based activities to forest-dependent rural households' income and highlighted key socioeconomic characteristics in Wolaita, Ethiopia. We collected demographic, socioeconomic, and forest use data using a semi-structured questionnaire survey of 384 households, and surveyed markets to determine the prices of forest products for the valuation of forest use in three districts. We used descriptive statistics, the Chi-square test, the two-sample t-test, and one-way ANOVA to analyze the data. Findings showed that all respondents were engaged in one way or another in forest-based activities, while 84.6 % were involved in farming. The annual average income from forest products was 252.7US per household, contributing to 28.1 % of the total yearly average income (989.4US$), and 38.3 % relative forest income (RFI). Annual mean household income from crops was 648.1US$. Average annual household income from grazing, charcoal, firewood, woodcutting, cut-and-carry, NTFP, seed selling, medicinal plants, and seedling selling was 77.3 %, 58.4 %, 50.2 %, 24.1 %, 18.5 %, 12.7 %, 7.1 %, 6.8 % and 5.4 % of the total annual mean income, respectively revealing a significant difference (p < 0.05). Family size, farmland size, distance to the forest, and occupation were the determinants of the contribution of forest products to household income. In general, poor households derive the highest relative forest income implying high dependence of the poorer on forest resources in the study area. The findings provide useful information for developing sustainable forest management policies and strategies to enhance the economic and ecological benefits of forests, and highlight the need for funding projects to pay attention to the specific household variables that affect forest use. Mainstreaming conservation activities in development sectors and integrating development and conservation projects may improve the livelihood of the low-income family in the region.

Conservation implications of mapping the potential distribution of an Ethiopian endemic versatile medicinal plant, Echinops kebericho Mesfin.

Echinops kebericho is a narrow-range multipurpose medicinal plant confined to Ethiopia. Intense land use change and overharvesting for traditional medicine have resulted in narrow distributions of its populations. It is a threatened species with a decreasing population trend. This study aims to map its potential distribution, which is key to guide conservation efforts and sustainable use. We modeled the potential distribution of E. kebercho using the maximum entropy model (MaxEnt) employing 11 less correlated predictor variables by calibrating the model at two complexity levels and replicating each model 10 times using a cross validation technique. We projected the models into the whole of Ethiopia and produced binary presence-absence maps by classifying the average map from both complexity levels applying three threshold criteria and ensembling the resulting maps into one for the final result. We mapped suitable habitat predicted with high certainty and identified local districts where E. kebericho can be cultivated or introduced to enhance its conservation. We estimated that E.kebercho has about 137,925 km2 of suitable habitat, mainly concentrated in the western highlands of the Ethiopian mountains. Our models at both complexity levels had high average performances, AUC values of 0.925 for the complex model and 0.907 for the simpler model. The variations in performance among the 10 model replicates were not remarkable, an AUC standard deviation of 0.040 for complex and 0.046 for simple model. Although E. kebericho is locally confined, our models predicted that it has a remarkably wider potential distribution area. We recommend introducing E. kebericho to these areas to improve its conservation status and tap its multiple benefits on a sustainable basis. Locally confined threatened plants and animals likely have wider potential distributions than their actual distributions and thus similar methodology can be applied for their conservation.

Ethnobotanical investigation of medicinal plants in Buska Mountain range, Hamar district, Southwestern Ethiopia.

BACKGROUND: Despite the fact that ethnobotanical studies have been conducted in various parts of Ethiopia, compared with the existence of the multitude and diverse ethnic groups and their associated traditional knowledge, the studies are not comprehensive enough for all the localities and communities in the country. This is also true for the Hamar community of Southwestern Ethiopia, who are totally dependent on plants and plant products for their livelihood. Hence, this investigation was done to identify and record medicine plants and the native wisdom of the community in the area. METHODS: Three hundred twenty six (326) informants were selected from the 12 lowest governmental units (Kebeles) applying Cochran's formula through stratified random sampling technique. From the total informants, 74 (48 males and 26 females) were purposively selected for in-depth discussion. Semi-structured interviews, focus group discussions, guided field walks as well as market surveys were used for data collection. Standard ethnobotanical analytical tools comprising ranking and comparison were used for the analysis. Preference ranking, pair-wise comparison, informant consensus factor, direct matrix ranking, Cultural Significance Index (CSI) and Jaccard's similarity coefficient (JCS) as well as Analysis of Variances (ANOVA), applying SPSS (version 20) were computed. RESULTS: A total of 145 species practical to cure about 72 ailments of livestock and humans were recorded. Families Fabaceae (with 22% of species), Asteraceae (11%), and Lamiaceae (9%) were discovered as the most dominant families in the area. Shrubs contributed the most (40%) to the growth forms followed by herbs (26.5%). Fresh leaves of the plants were parts that are used most frequently in the area. The highest ICF value (0.94) was recorded for reproductive problem categories. There was a relatively very high dependence of the community on plants and plant products together with a hoarded indigenous knowledge in the area that positively correlated with age (r = 0.82). CONCLUSION: The study's findings revealed that Buska Mountain range is a home for highly diverse and most dependable plant species and associated indigenous knowledge. However, because of the realized environmental threats in the area, the conservation efforts of the community should be invigorated and supported in order to sustain the biodiversity in general and the medicinal plant species in particular.

Herbal medicine used by the community of Koneba district in Afar Regional State, Northeastern Ethiopia.

BACKGROUND: Pastoral communities of the Afar people in northeastern Ethiopia use medicinal plants for various health problems. However, very limited scientific documents are found addressing ethnomedicinal knowledge of the community. OBJECTIVE: This study aimed at documenting herbal medicine and the associated knowledge from Koneba district of Afar Regional State, Ethiopia. METHODS: Purposive sampling method was used to select study sites and key informants. General informants were selected through simple random sampling methods. Semi-structured interviews and guided field walk were used to collect data while Informant Consensus Factor (ICF), Fidelity Level (FL) and Preference Ranking were used to analyze and verify data. RESULTS: A total of 67 medicinal plant species used to treat humans and livestock ailments were recorded and collected. Thirteen medicinal plant species were mentioned as effective medicine against snake bite (ICF; 0.68) while nine species used to treat malaria, common cold and fever (ICF: 0.67). Cyphostemma adenocaule (Steud. ex A.Rich.) Desc. ex Wild & R.B.Drumm. was the most preferred species used to combat snakebite, which was prevalent in the area. CONCLUSION: Snake bite, malaria, common cold and fever are common health problems in the study area. Efficient use of herbal medicine has minimized the impact of these diseases.

The Genetic Diversity of Enset (Ensete ventricosum) Landraces Used in Traditional Medicine Is Similar to the Diversity Found in Non-medicinal Landraces.

Enset (Ensete ventricosum) is a multipurpose crop extensively cultivated in southern and southwestern Ethiopia for human food, animal feed, and fiber. It has immense contributions to the food security and rural livelihoods of 20 million people. Several distinct enset landraces are cultivated for their uses in traditional medicine. These landraces are vulnerable to various human-related activities and environmental constraints. The genetic diversity among the landraces is not verified to plan conservation strategy. Moreover, it is currently unknown whether medicinal landraces are genetically differentiated from other landraces. Here, we characterize the genetic diversity of medicinal enset landraces to support effective conservation and utilization of their diversity. We evaluated the genetic diversity of 51 enset landraces, of which 38 have reported medicinal value. A total of 38 alleles across the 15 simple sequence repeat (SSR) loci and a moderate level of genetic diversity (He = 0.47) were detected. Analysis of molecular variation (AMOVA) revealed that only 2.4% of the total genetic variation was contributed by variation among the medicinal and non-medicinal groups of landraces, with an FST of 0.024. A neighbor-joining tree showed four separate clusters with no correlation to the use-values of the landraces. Except for two, all "medicinal" landraces with distinct vernacular names were found to be genetically different, showing that vernacular names are a good indicator of genetic distinctiveness in these specific groups of landraces. The discriminant analysis of the principal components also confirmed the absence of distinct clustering between the two groups. We found that enset landraces were clustered irrespective of their use-value, showing no evidence for genetic differentiation between the enset grown for 'medicinal' uses and non-medicinal landraces. This suggests that enset medicinal properties may be restricted to a more limited number of genotypes, might have resulted from the interaction of genotype with the environment or management practice, or partly misreported. The study provides baseline information that promotes further investigations in exploiting the medicinal value of these specific landraces.

Medicinal plant use practice in four ethnic communities (Gurage, Mareqo, Qebena, and Silti), south central Ethiopia.

BACKGROUND: Ethnic groups throughout the world have developed their own cultures expressed in the form of customs, taboos, and traditional healthcare systems. Traditional medicine system is one of the widespread cultures known throughout the world which is very much tied to cultural practices of the community or ethnic group. Medicinal plant treasure found in Gurage and Silti zones remained poorly characterized and understood. Therefore, this study was conducted in four ethnic groups: three from Gurage zone (Gurage, Qebena, and Mareqo) and one from Silti zone (Silti) which have lived in close proximity and contact for many centuries in the respective zones. In the present study, unique and shared cultural elements in connection to traditional herbal medicine were examined through investigation of the diversity of medicinal plants. Moreover, attempts have been made to determine similarities among the society in the medicinal plants they have used in general and in medicinal plant species considered culturally most important. METHODS: In a study that involved 320 randomly sampled informants, semi-structured interviews, focus group discussions, and participant observation were used and qualitative and quantitative data were collected. Descriptive statistics, rank order priority (ROP), informant consensus factor, Jaccard similarity coefficient, and clustering were used for data analysis. RESULTS: A total of 244 medicinal plant species and a fungal species used to treat human and/or livestock ailments were documented. The number of plants (80 plants, 33 %) with ROP value greater than 50% were considerably fewer than that of plants with ROP < 50% (164, 67 %). Jaccard similarity index and clustering analysis for all cited plants, among the respective studied districts, indicated that grouping generally followed the existing ethnic origin. On the contrary, clustering based on culturally important medicinal plant species (80 plant species, score ROP ≥ 50%) showed the influence of proximity and geographical orientation rather than ethnic relation. CONCLUSIONS: Culturally, most important plants (80 spp.) are widely used and best shared with nearby communities and this could imply current (new) knowledge being practiced in the communities. This knowledge must be documented and better utilized in a modern way including modernized use of traditional medicinal plants.

An ethnobotanical study of medicinal plants in Sheka Zone of Southern Nations Nationalities and Peoples Regional State, Ethiopia.

BACKGROUND: People's classification, management, and use of plants represent attempt to attracting people from different academic disciplines. Many countries use traditional medicine for their primary healthcare system. Medicinal plants have been important components of healthcare systems since the time immemorial. The objective of this research was to document and analyze traditional medicinal plants used by the Sheka people and associated ethnobotanical knowledge. METHODS: Data was collected by administering pre-prepared semi-structured interview items to 414 informants. Market surveys, group discussion, and guided field walk were used. Data were analyzed using descriptive and inferential statistics; determination of informant consensus factor, fidelity level, as well as ranking and scoring. RESULTS: A total of 266 plant species belonging to 192 genera and 74 families were identified. About 204 (77%) of the medicinal plants were used to treat human health problems. Only ten (4%) were used to treat livestock health problems and 52 (19%) of them were used to treat both human and livestock health problems. Croton macrostachyus, Prunus africana, Peperomia retusa, Lobelia giberroa, and Celosia schweinfurthiana were found to be high ranking medicinal plants against gastrointestinal problems based on simple preference ranking. CONCLUSION: Very high number of medicinal plant species recorded from the study area indicates that vegetation of Sheka is reservoir of medicinal plants. Hence, the area needs attention for medicinal plant conservation priorities. Plant parts used as medicines also play vital role in the entire medicinal plant life cycle. Therefore, it is useful to consider harvesting impacts. Except well-experienced traditional healers, people of the study area use the medicinal plants haphazardly. There may be high risk of being victims of dosage and improper usage. High ranking medicinal plants are candidates for further phytochemical profiling, drug research, and development.

An iconic traditional apiculture of park fringe communities of Borena Sayint National Park, north eastern Ethiopia.

BACKGROUND: Traditional apiculture has been practised in Ethiopia over a long historical period and still remains a benign means to extract direct benefits from natural ecosystems. While its contribution to economic development and watershed protection is increasingly recognized its cultural significance is however, seldom noticed. This study was conducted using an ethnobotanical study approach to document the honey bee flora and associated indigenous knowledge of local communities in Borena Sayint National Park (BSNP), north eastern Ethiopia. METHODS: Data were collected from 170 informants through semi-structured interviews and guided field walks, focus group discussion with 37 informants and 14 key informants and analyzed using standard analytical tools including ranking, comparisons and multivariate analyses. RESULTS: In total, 152 bee forage species in 133 genera and 74 families were documented. The Asteraceae and Rosaceae were represented with six species each over the other plant families. Percentage of mentions per species ranged between 76.9 and 13.5% for the most salient bee forage species. Dombeya torrida, Erica arborea, and Olinia rochetiana captured high community consensus as measured by rank order of popularity and designated as local appellation names of honey. Cluster analysis of priority ranking data showed relationships between key informants with respect to preferences, but ordination analysis did not indicate environmental proximity as a determinant of their responses. Five honey harvesting seasons occur each corresponding to the floral calendar of a dominant bee forage species that stipulate relocation of hives to appropriate locations within the national park. CONCLUSION: The apicultural tradition is iconic with economic value and forming part of the local peoples' cultural identity apt to be preserved as a bequest for posterity.

In vitro antimicrobial activity of plants used in traditional medicine in Gurage and Silti Zones, south central Ethiopia.

BACKGROUND: To overcome the escalating problems associated with infectious diseases and drug resistance, discovery of new antimicrobials is crucial. The present study aimed to carry out in vitro antimicrobial analysis of 15 medicinal plant species selected according to their traditional medicinal uses in Gurage and Silti Zones, south central Ethiopia. METHODS: Ethanol extracts of various plant parts were investigated for their antimicrobial activity against 20 bacterial and one yeast strains. The minimum inhibitory concentration (MIC) was determined by broth microdilution method. RESULTS: Asparagus africanus, Guizotia schimperi, Lippia adoensis var. adoensis and Premna schimperi were active against Candida albicans, Enterococcus faecalis and Staphylococcus aureus at a concentration of 512 µg/ml or lower. Strong antibacterial activity (MIC≥128 µg/ml) was observed for G. schimperi extract against 17 resistant and sensitive Staphylococcus strains, at a concentration comparable to standard antibiotics. Moreover, this extract showed higher antibacterial activity for the test against S. aureus ATCC 33591, ATCC 33592, SA3 and SA5 strains (128-256 µg/ml) than oxacillin (512 µg/ml). CONCLUSIONS: The study revealed in vitro antibacterial activity of plants used in folk medicine in south central Ethiopia. The usefulness of these plants, in particular of G. schimperi, should be confirmed through further phytochemical and toxicity analyses.

Evolutionary history and leaf succulence as explanations for medicinal use in aloes and the global popularity of Aloe vera.

BACKGROUND: Aloe vera supports a substantial global trade yet its wild origins, and explanations for its popularity over 500 related Aloe species in one of the world's largest succulent groups, have remained uncertain. We developed an explicit phylogenetic framework to explore links between the rich traditions of medicinal use and leaf succulence in aloes. RESULTS: The phylogenetic hypothesis clarifies the origins of Aloe vera to the Arabian Peninsula at the northernmost limits of the range for aloes. The genus Aloe originated in southern Africa ~16 million years ago and underwent two major radiations driven by different speciation processes, giving rise to the extraordinary diversity known today. Large, succulent leaves typical of medicinal aloes arose during the most recent diversification ~10 million years ago and are strongly correlated to the phylogeny and to the likelihood of a species being used for medicine. A significant, albeit weak, phylogenetic signal is evident in the medicinal uses of aloes, suggesting that the properties for which they are valued do not occur randomly across the branches of the phylogenetic tree. CONCLUSIONS: Phylogenetic investigation of plant use and leaf succulence among aloes has yielded new explanations for the extraordinary market dominance of Aloe vera. The industry preference for Aloe vera appears to be due to its proximity to important historic trade routes, and early introduction to trade and cultivation. Well-developed succulent leaf mesophyll tissue, an adaptive feature that likely contributed to the ecological success of the genus Aloe, is the main predictor for medicinal use among Aloe species, whereas evolutionary loss of succulence tends to be associated with losses of medicinal use. Phylogenetic analyses of plant use offer potential to understand patterns in the value of global plant diversity.

Medicinal plants potential and use by pastoral and agro-pastoral communities in Erer Valley of Babile Wereda, Eastern Ethiopia.

BACKGROUND: Ethiopian plants have shown remarkably effective medicinal values for many human and livestock ailments. Some research results are found on medicinal plants of the south, south west, central, north and north western parts of Ethiopia. However, there is lack of data that quantitatively assesses the resource potential and the indigenous knowledge on use and management of medicinal plants in eastern Ethiopia. The main thrust of the present ethnobotanical study centres around the potential and use of traditional medicinal plants by pastoral and agro-pastoral communities in Babile Wereda (district) of eastern Ethiopia. The results can be used for setting up of conservation priorities, preservation of local biocultural knowledge with sustainable use and development of the resource. MATERIALS AND METHODS: Fifty systematically selected informants including fifteen traditional herbalists (as key informants) participated in the study. Semi-structured interviews, discussions and guided field walk constituted the main data collection methods. Techniques of preference ranking, factor of informant consensus and Spearman rank correlation test were employed in data analysis. Medicinal plant specimens were collected, identified and kept at the National Herbarium (ETH) of Addis Ababa University and Haramaya University Herbarium. RESULTS: Fifty-one traditional medicinal plant species in 39 genera and 28 families were recorded, constituting 37% shrubs, 29% trees, 26% herbs, 6% climbers and 2% root parasites. Leaves contributed to 35.3% of the preparations, roots (18.8%) and lower proportions for other parts. Formulations recorded added to 133 remedies for 54 human ailments, in addition to some used in vector control. The majority of remedies were the juice of single species, mixtures being generally infrequent. Aloe pirottae, Azadirachta indica and Hydnora johannis were the most cited and preferred species. Aloe pirottae, a species endemic to Ethiopia, is valued as a remedy for malaria, tropical ulcer, gastro-intestinal parasites, gallstone, eye diseases and snake bite. The jel extracted from dried and ground plant material, called SIBRI (Oromo language), was acclaimed as a cleaner of the human colon. Concoction made from leaf, seed and flower of Azadirachta indica was given for treatment of malaria, fungal infections and intestinal worms. Root preparations from Hydnora johannis were prescribed as remedy for diarrhoea, haemorrhage, wound and painful body swelling, locally called GOFLA (Oromo language). CONCLUSIONS: The study documented many well known and effective medicinal species of relevance for human healthcare, including for the treatment of malaria which is rampant in the area as it is in many parts of Ethiopia. This underscores the importance of the traditional medicinal plants for the people living in the area and the potential of the resource for development. Consequently, the study area deserves urgent conservation priority coupled with mechanisms for the protection of the associated indigenous medical lore as well as development and effective use of the medicinal plant resource.

An ethnobotanical study of medicinal plants in Wonago Woreda, SNNPR, Ethiopia.

BACKGROUND: Medicinal plants are the integral part of the variety of cultures in Ethiopia and have been used over many centuries. Hence, the aim of this study is to document the medicinal plants in the natural vegetation and home gardens in Wonago Woreda, Gedeo Zone, Southern Nations, Nationalities and Peoples Regional State (SNNPR). MATERIALS AND METHODS: Thirty healers were selected to collect data on management of medicinal plants using semi-structured interview, group discussion, and field observation. The distribution of plant species in the study areas was surveyed, and preference ranking, direct matrix ranking, priority ranking of factors and Informant consensus factor (ICF) were calculated. RESULTS: The informants categorized the vegetation into five community types based on plant density and associated landform: 'Raqqa', 'Hakka cadanaba', 'Mancchha', 'Bullukko', and 'Wodae gido'. 155 plant species were collected from the natural vegetation and 65 plant species from the home gardens ('Gattae Oduma'). Seventy-two plant species were documented as having medicinal value: Sixty-five (71%) from natural vegetation and 27 (29%) from home gardens. Forty-five (62%) were used for humans, 15(21%) for livestock and 13(18%) for treating both human and livestock ailments: 35 (43.2%) were Shrubs, 28(34.5%) herbs, 17 (20.9%) trees and 1(1.2%) climbers. The root (35.8%) was the most commonly used plant part. The category: malaria, fever and headache had the highest 0.82 ICF. Agricultural expansion (24.4%) in the area was found to be the main threat for medicinal plants followed by fire wood collection (18.8%). Peoples' culture and spiritual beliefs somehow helped in the conservation of medicinal plants. CONCLUSION: Traditional healers still depend largely on naturally growing plant species and the important medicinal plants are under threat. The documented medicinal plants can serve as a basis for further studies on the regions medicinal plants knowledge and for future phytochemical and pharmacological studies.